{"id":7351,"date":"2022-04-19T14:32:26","date_gmt":"2022-04-19T14:32:26","guid":{"rendered":"https:\/\/aboutsmallruminants.com\/?p=7351"},"modified":"2022-04-22T10:58:02","modified_gmt":"2022-04-22T10:58:02","slug":"agalactie-contagieuse-diagnostic","status":"publish","type":"post","link":"https:\/\/aboutsmallruminants.com\/fr\/agalactie-contagieuse-diagnostic\/","title":{"rendered":"Agalactie contagieuse : comment d\u00e9tectons-nous le probl\u00e8me ?"},"content":{"rendered":"
L’agalactie contagieuse<\/a> est une maladie tr\u00e8s importante chez les brebis et les ch\u00e8vres, en particulier dans l\u2019industrie laiti\u00e8re. Pour cette raison, il est vital d’\u00e9tablir un bon diagnostic afin de pouvoir contr\u00f4ler et pr\u00e9venir la maladie, et par-dessus tout, d\u2019\u00e9viter les co\u00fbts<\/a> qui s’ensuivent.
\n<\/strong><\/div>\n

 <\/p>\n

Difficult\u00e9s du diagnostic<\/h2>\n

D\u2019une mani\u00e8re g\u00e9n\u00e9rale, Mycoplasma<\/strong> est sous-diagnostiqu\u00e9<\/a>\u00a0et le diagnostic diff\u00e9rentiel<\/a> de la mammite<\/strong> n\u2019est habituellement pas pris en compte dans le cas des brebis et des ch\u00e8vres, mais dans les troupeaux end\u00e9miques<\/strong>, il peut s\u2019agir de la deuxi\u00e8me, voire de la troisi\u00e8me cause de la maladie.<\/p>\n

L\u2019agalactie contagieuse<\/strong> pr\u00e9sente diff\u00e9rentes caract\u00e9ristiques qui rendent le diagnostic difficile<\/strong>\u00a0:<\/p>\n

\u25cf Variabilit\u00e9 des signes cliniques<\/strong>\u00a0: mammite<\/a>, arthrite, k\u00e9ratoconjonctivite, pneumonie.<\/p>\n

\u25cf Variabilit\u00e9 de l\u2019\u00e9tiologie<\/strong>: M. agalactiae<\/em> (le plus pr\u00e9valent), M. mycoides<\/em>, M. capricolum<\/em> et M. putrefaciens<\/em>.<\/p>\n

\u25cf Les diff\u00e9rentes esp\u00e8ces<\/strong> de Mycoplasma partagent des caract\u00e9ristiques g\u00e9n\u00e9tiques semblables, ce qui rend leur identification difficile.<\/p>\n

\u25cf Dans les troupeaux end\u00e9miques<\/strong>, des ann\u00e9es peuvent s\u2019\u00e9couler sans qu\u2019aucun sympt\u00f4me n\u2019apparaisse.<\/p>\n

\u25cf L’excr\u00e9tion<\/a> peut \u00eatre intermittente.<\/p>\n

\u25cf Les animaux infect\u00e9s de fa\u00e7on chronique<\/strong> sont g\u00e9n\u00e9ralement n\u00e9gatifs au niveau s\u00e9rologique.<\/p>\n

La technique ELISA est utile pour d\u00e9terminer l\u2019\u00e9tat de sant\u00e9 du troupeau.<\/span><\/strong><\/p><\/blockquote>\n

 <\/p>\n

Comment \u00e9tablir un bon diagnostic ?<\/h2>\n

Le premier point important est de pr\u00e9lever des \u00e9chantillons appropri\u00e9s<\/strong>\u00a0: lait, sang, liquide synovial et \u00e9couvillonnages nasal, conjonctival et auriculaire.<\/p>\n

Il est recommand\u00e9 d\u2019analyser les \u00e9chantillons de lait individuels<\/strong> en m\u00eame temps que des \u00e9chantillons de lait en citerne en vrac simultan\u00e9s<\/a>. Des pr\u00e9l\u00e8vements doivent \u00eatre effectu\u00e9s de mani\u00e8re r\u00e9guli\u00e8re sur l\u2019exploitation, tous les 3 mois<\/strong> si possible.<\/p>\n

\"\"
Fig. 1. L\u2019une des meilleures mani\u00e8res de d\u00e9tecter les mycoplasmes dans le troupeau est de pr\u00e9lever du lait de citerne en vrac, en particulier dans les zones end\u00e9miques.<\/figcaption><\/figure>\n

En ce qui concerne les techniques<\/strong>, celles le plus fr\u00e9quemment utilis\u00e9es sont\u00a0:<\/p>\n

\u25cf ELISA:<\/strong> il s’agit d’une bonne option, mais elle peut ne pas \u00eatre tr\u00e8s sensible<\/strong> dans les troupeaux end\u00e9miques en raison de l\u2019absence d\u2019anticorps d\u00e9tectables. En outre, des r\u00e9actions crois\u00e9es entre des esp\u00e8ces de Mycoplasma<\/em> non pathog\u00e8nes peuvent donner des r\u00e9sultats faux positifs<\/strong>. Un autre probl\u00e8me est que la technique ne distingue pas les anticorps vaccinaux<\/strong> de ceux dus \u00e0 une infection.<\/p>\n

La technique ELISA est utile pour d\u00e9terminer l\u2019\u00e9tat de sant\u00e9 du troupeau.<\/span><\/strong><\/p><\/blockquote>\n

\u25cf PCR<\/a>:<\/strong> il s\u2019agit de la technique la plus rapide et la plus sensible<\/strong> pour confirmer l\u2019infection, et celle qui est normalement la plus r\u00e9pandue. Elle permet l\u2019identification du pathog\u00e8ne<\/strong> et, du fait de son haut degr\u00e9 de sp\u00e9cificit\u00e9<\/strong>, elle est capable de d\u00e9tecter diff\u00e9rentes esp\u00e8ces de mycoplasma.<\/p>\n

Pour un diagnostic d\u00e9finitif, le pathog\u00e8ne doit \u00eatre isol\u00e9 et identifi\u00e9.<\/span><\/strong><\/p><\/blockquote>\n

D\u2019autres techniques sont \u00e9galement disponibles, comme : la culture et les tests biochimiques, la fixation du compl\u00e9ment et l\u2019immunofluorescence.<\/strong><\/p>\n

 <\/p>\n

Conclusions<\/h2>\n

\u25cf L\u2019agalactie contagieuse<\/strong> est une maladie grave qui doit \u00eatre diagnostiqu\u00e9e.<\/p>\n

\u25cf Le diagnostic de laboratoire<\/strong> est essentiel.<\/p>\n

\u25cf Une fois le probl\u00e8me identifi\u00e9, il peut \u00eatre contr\u00f4l\u00e9 par la vaccination<\/a> du troupeau.<\/p>\n

 <\/p>\n

 <\/p>\n

Article \u00e9crit par: <\/strong>
\nTania Per\u00e1lvarez Puerta. Gestionnaire de produit de l\u2019entreprise, unit\u00e9 des petits ruminants \u2013 HIPRA<\/p>\n","protected":false},"excerpt":{"rendered":"

L’agalactie contagieuse est une maladie tr\u00e8s importante chez les brebis et les ch\u00e8vres, en particulier dans l\u2019industrie laiti\u00e8re. Pour cette raison, il est vital d’\u00e9tablir un bon diagnostic afin de pouvoir contr\u00f4ler et pr\u00e9venir la maladie, et par-dessus tout, d\u2019\u00e9viter les co\u00fbts qui s’ensuivent.   Difficult\u00e9s du diagnostic D\u2019une mani\u00e8re g\u00e9n\u00e9rale, Mycoplasma est sous-diagnostiqu\u00e9\u00a0et le […]<\/p>\n","protected":false},"author":10,"featured_media":7331,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[39],"tags":[],"class_list":["post-7351","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-mammites"],"yoast_head":"\nAgalactie contagieuse : comment parvenir \u00e0 la diagnostiquer ?<\/title>\n<meta name=\"description\" content=\"L\u2019agalactie contagieuse est une maladie tr\u00e8s importante chez les brebis et les ch\u00e8vres, en particulier dans l\u2019industrie laiti\u00e8re.\" \/>\n<meta name=\"robots\" content=\"index, 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